rat mmp 9  (Elabscience Biotechnology)

Journal: International Journal of Molecular Medicine

Article Title: Bumetanide-blocked SLC12A2 exerts a protective effect in experimental diabetic retinopathy

doi: 10.3892/ijmm.2026.5774

Figure Lengend Snippet: Effect of SLC12A2 knockdown on inflammatory factor release and blood-retinal barrier in HG-treated rat retinal microvascular endothelial cells. (A) Levels of VEGF, IL-1β, IL-6 and TNF-α detected by ELISA. (B) Immunofluorescence staining showing the ZO-1 expression in each group. (C) Levels of MMP2 and MMP9 detected by commercial kits. Data from three independent experiments are indicated as the mean ± SD (n=3). * P<0.05 and ** P<0.01. HG, high glucose; VEGF, vascular endothelial growth factor; IL-1β, interleukin-1Beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor-alpha; ZO-1, zonula occludens-1; MMP, matrix metalloproteinase; n.s., not significant.

Article Snippet: In the present study, kits for MMP2 (cat. no. E-EL-R0618) and MMP9 (cat. no. E-EL-R3021; both from Elabscience Biotechnology, Inc.) were employed to detect the activity of these two factors in cell supernatants.

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Expressing

Journal: Molecular Medicine Reports

Article Title: Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air-blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF-κB/IκBα pathway

doi: 10.3892/mmr.2026.13797

Figure Lengend Snippet: Injury of mucus hypersecretion and oxidative damage in all groups. (A) AB-PAS staining images of the airway in all groups (AB-PAS; ×200 magnification and ×400 magnification). Arrows indicate goblet cells. (B) The expression level of MUC5AC and MUC5B in the airway tested using immunohistochemistry (×200 magnification). (C) The expression of IL-6, IL-10, TNF-α, T-SOD, MDA, MMP-9 and TIMP-1 in lung tissue and serum of rats in all groups. The values are expressed as mean ± SE; (n=8). *P<0.05 and **P<0.01 vs. normal group. AB-PAS, Alcian blue-periodic acid-schiff; MUC5AC, mucoprotein-5AC; MUC5B, mucoprotein-5B; T-SOD, total superoxide dismutase; MDA, malondialdehyde; MMP-9, matrix metalloproteinase 9; TIMP-1, tissue inhibitor of metalloproteinases 2; IOD, integrated optical density.

Article Snippet: The secretion of IL-6 (Rat IL-6 ELISA Kit; cat. no. E-EL-R0015; Elabscience Bionovation Inc.), IL-10 (Rat IL-10 ELISA Kit; cat. no. E-EL-R0016; Elabscience Bionovation Inc.), malondialdehyde (MDA) (MDA assay kit; cat. no. A003-1-2; Nanjing Jiancheng Bioengineering Institute), total superoxide dismutase (T-SOD) (SOD assay kit; cat. no. A001-3-2; Nanjing Jiancheng Bioengineering Institute), MMP-9 (Rat MMP-9 ELISA Kit; cat. no. E-EL-R3021; Elabscience Bionovation Inc.) and tissue Inhibitors of metalloproteinase 1 (TIMP-1 ELISA Kit; cat. no. E-EL-R0540; Elabscience Bionovation Inc.) in the lung tissue homogenate was measured using ELISA kits, following the manufacturer's instructions.

Techniques: Staining, Expressing, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Elevated MMP9 Expression—A Potential In Vitro Biomarker for COMPopathies

doi: 10.3390/ijms262412070

Figure Lengend Snippet: MMP9 is upregulated in cell models of COMPopathies. ( A ) Volcano plot of differentially expressed genes. ( B ) Validation of differential expression (log 2 fold change) of MMP9 , MMP1 , GALNT18 and SOX9 by qRT-PCR. Means and standard deviations of 3 replicates are shown. Student’s t -test was used to determine p -values. ( C ) Representative Western blot of cell lysates and conditioned media of wild-type (WT) and CY (p.C312Y, MED), DN (p.D385N, MED), DH (p.D473H, PSACH), GR (p.G440R, PSACH) and DY (p.D511Y, PSACH)-overexpressing HT1080 cells 72 h after confluency. COMPs were detected via the C-terminal GFP-tag. GAPDH was used as a loading control. ( D ) MMP9 expression was assessed by qRT-PCR 72 h after confluency. 18S was used as a housekeeping gene. Means and standard deviations of 3 replicates are shown; p -values were determined using ANOVA and Tukey post hoc tests.

Article Snippet: The level of MMP9 in the resulting serum was then deduced using the R&D Systems Mouse Total MMP9 Quantikine ELISA kit (Biotechne Ltd., Abingdon, UK) according to the manufacturer’s instructions.

Techniques: Biomarker Discovery, Quantitative Proteomics, Quantitative RT-PCR, Western Blot, Control, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Elevated MMP9 Expression—A Potential In Vitro Biomarker for COMPopathies

doi: 10.3390/ijms262412070

Figure Lengend Snippet: Expression of mutant matrilin-3 (MED) does not drive MMP9 expression. ( A ) Representative Western blot of flag-tagged matrilin-3 and BiP protein levels in untransfected (NC) WT or VD transfected cells 48 h after transfection. GAPDH was used as loading control. Means and standard deviations of 3 experiments are shown. ( B ) XBP1 splicing was analysed in wild-type and mutant VD matrilin-3 overexpressing HT1080 cells using RT-PCR and agarose gel electrophoresis. Means and standard deviations of 3 replicates are shown. ( C ) Analysis of HSPA5 and MMP9 expression by qRT-PCR. 18S was used as a housekeeping gene. Means and standard deviations of 3 replicates are shown. ( B , C ) Student’s t -test was used to determine p -values.

Article Snippet: The level of MMP9 in the resulting serum was then deduced using the R&D Systems Mouse Total MMP9 Quantikine ELISA kit (Biotechne Ltd., Abingdon, UK) according to the manufacturer’s instructions.

Techniques: Expressing, Mutagenesis, Western Blot, Transfection, Control, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: Elevated MMP9 Expression—A Potential In Vitro Biomarker for COMPopathies

doi: 10.3390/ijms262412070

Figure Lengend Snippet: MMP9 serum levels are not upregulated in the PSACH mouse model. Serum samples from ( A ) female wild-type (N = 5) and D469del COMP PSACH mice (N = 13) and ( B ) male wild-type (N = 7) or p.D469del COMP PSACH mice (N = 12) were analysed by ELISA. ( C ) MMP9 serum levels in wild-type (N = 12) and D469del COMP PSACH mice (N = 25) of both sexes. Student’s t -test was applied to determine p -values.

Article Snippet: The level of MMP9 in the resulting serum was then deduced using the R&D Systems Mouse Total MMP9 Quantikine ELISA kit (Biotechne Ltd., Abingdon, UK) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay